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primary gbm culture  (Addgene inc)


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    Structured Review

    Addgene inc primary gbm culture
    Expansion microscopy improves antibody penetration into tumor spheroids. Central sections of expanded <t>GBM#18</t> tumor spheroids expressing a <t>cytosolic</t> <t>tdTomato</t> fluorescent protein (A) , line profile of fluorescent intensity through the center of a cleared or expanded spheroid (endogenous tdTomato signal) (B) , line profile of fluorescent intensity through the center of a cleared or expanded spheroid (anti-tdTomato immuno-stain signal) (C) . Fluorescence (AU) was normalized and distance of the expanded spheroids was normalized to that of the cleared spheroid. Scale bar: 50 μm (cleared), 200 μm (expanded).
    Primary Gbm Culture, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+gbm+culture/pmc07543521-26-5-20?v=Addgene+inc
    Average 92 stars, based on 20 article reviews
    primary gbm culture - by Bioz Stars, 2026-06
    92/100 stars

    Images

    1) Product Images from "High-Resolution Imaging of Tumor Spheroids and Organoids Enabled by Expansion Microscopy"

    Article Title: High-Resolution Imaging of Tumor Spheroids and Organoids Enabled by Expansion Microscopy

    Journal: Frontiers in Molecular Biosciences

    doi: 10.3389/fmolb.2020.00208

    Expansion microscopy improves antibody penetration into tumor spheroids. Central sections of expanded GBM#18 tumor spheroids expressing a cytosolic tdTomato fluorescent protein (A) , line profile of fluorescent intensity through the center of a cleared or expanded spheroid (endogenous tdTomato signal) (B) , line profile of fluorescent intensity through the center of a cleared or expanded spheroid (anti-tdTomato immuno-stain signal) (C) . Fluorescence (AU) was normalized and distance of the expanded spheroids was normalized to that of the cleared spheroid. Scale bar: 50 μm (cleared), 200 μm (expanded).
    Figure Legend Snippet: Expansion microscopy improves antibody penetration into tumor spheroids. Central sections of expanded GBM#18 tumor spheroids expressing a cytosolic tdTomato fluorescent protein (A) , line profile of fluorescent intensity through the center of a cleared or expanded spheroid (endogenous tdTomato signal) (B) , line profile of fluorescent intensity through the center of a cleared or expanded spheroid (anti-tdTomato immuno-stain signal) (C) . Fluorescence (AU) was normalized and distance of the expanded spheroids was normalized to that of the cleared spheroid. Scale bar: 50 μm (cleared), 200 μm (expanded).

    Techniques Used: Microscopy, Expressing, Immunostaining, Fluorescence



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    Addgene inc primary gbm culture
    Expansion microscopy improves antibody penetration into tumor spheroids. Central sections of expanded <t>GBM#18</t> tumor spheroids expressing a <t>cytosolic</t> <t>tdTomato</t> fluorescent protein (A) , line profile of fluorescent intensity through the center of a cleared or expanded spheroid (endogenous tdTomato signal) (B) , line profile of fluorescent intensity through the center of a cleared or expanded spheroid (anti-tdTomato immuno-stain signal) (C) . Fluorescence (AU) was normalized and distance of the expanded spheroids was normalized to that of the cleared spheroid. Scale bar: 50 μm (cleared), 200 μm (expanded).
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    Image Search Results


    Expansion microscopy improves antibody penetration into tumor spheroids. Central sections of expanded GBM#18 tumor spheroids expressing a cytosolic tdTomato fluorescent protein (A) , line profile of fluorescent intensity through the center of a cleared or expanded spheroid (endogenous tdTomato signal) (B) , line profile of fluorescent intensity through the center of a cleared or expanded spheroid (anti-tdTomato immuno-stain signal) (C) . Fluorescence (AU) was normalized and distance of the expanded spheroids was normalized to that of the cleared spheroid. Scale bar: 50 μm (cleared), 200 μm (expanded).

    Journal: Frontiers in Molecular Biosciences

    Article Title: High-Resolution Imaging of Tumor Spheroids and Organoids Enabled by Expansion Microscopy

    doi: 10.3389/fmolb.2020.00208

    Figure Lengend Snippet: Expansion microscopy improves antibody penetration into tumor spheroids. Central sections of expanded GBM#18 tumor spheroids expressing a cytosolic tdTomato fluorescent protein (A) , line profile of fluorescent intensity through the center of a cleared or expanded spheroid (endogenous tdTomato signal) (B) , line profile of fluorescent intensity through the center of a cleared or expanded spheroid (anti-tdTomato immuno-stain signal) (C) . Fluorescence (AU) was normalized and distance of the expanded spheroids was normalized to that of the cleared spheroid. Scale bar: 50 μm (cleared), 200 μm (expanded).

    Article Snippet: A498 renal carcinoma and a primary GBM culture #18 ( ) stably expressing tdTomato following lentiviral transduction with plasmid #32904 (Addgene) were used to produce tumor spheroids.

    Techniques: Microscopy, Expressing, Immunostaining, Fluorescence

    Cancer cell types, sources, and culture conditions

    Journal: Bioengineering & Translational Medicine

    Article Title: Polymeric nanoparticle‐based delivery of TRAIL DNA for cancer‐specific killing

    doi: 10.1002/btm2.10019

    Figure Lengend Snippet: Cancer cell types, sources, and culture conditions

    Article Snippet: JHGBM‐965 , Glioblastoma (GBM) Brain Tumor Initiating Cell (BTIC) primary culture , Human , Dr. Alfredo Quiñones‐Hinojosa, Department of Neurosurgery, Johns Hopkins University , DMEM/F12 (1:1) + B‐27 serum‐free supplement, 1% anti‐anti, 20 ng/ml bFGF, 20 ng/ml epidermal growth factor (EGF).

    Techniques: Cell Culture